• Western blot analysis of lysates from LOVO cells, using FZD5 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of A549 cells, using FZD5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-FZD5 antibody (461-510 aa) (STJ93146)

SKU:
STJ93146

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Frizzled-5 (461-510 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FZD5
Gene ID: 7855
Uniprot ID: FZD5_HUMAN
Immunogen Region: 461-510 aa
Specificity: Frizzled-5 Polyclonal Antibody detects endogenous levels of Frizzled-5 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human FZD5 at the amino acid range 461-510
Function Receptor for Wnt proteins. Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro).the in vivo situation may be different since not all of these are known to be coexpressed. In neurons, activation of WNT7A promotes formation of synapses. Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization.
Protein Name Frizzled-5
Fz-5
Hfz5
Fze5
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-4086398
Reactome: R-HSA-4608870
Reactome: R-HSA-4641262
Reactome: R-HSA-4641263
Reactome: R-HSA-5140745
Reactome: R-HSA-5340588
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Golgi Apparatus Membrane
Synapse
Perikaryon
Cell Projection
Dendrite
Axon
Localized At The Plasma Membrane And Also Found At The Golgi
Alternative Antibody Names Anti-Frizzled-5 antibody
Anti-Fz-5 antibody
Anti-Hfz5 antibody
Anti-Fze5 antibody
Anti-FZD5 antibody
Anti-C2orf31 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance