| Host: |
Goat |
| Applications: |
Pep-ELISA/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-FURIN/PCSK3 (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
IF-Strong expression of the protein seen in the plasma membrane and ER/cytoplasm of HeLa cells, and additionally the nucleus of U2OS cells. 10µg/mlELISA-antibody detection limit dilution 1:32000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
FURIN |
| Gene ID: |
5045 |
| Uniprot ID: |
FURIN_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_002560.1 |
| Immunogen Sequence: |
RTKRDVYQEPTDP |
| Post Translational Modifications | The inhibition peptide, which plays the role of an intramolecular chaperone, is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Following transport to the trans Golgi, a second cleavage within the inhibition propeptide results in propeptide dissociation and furin activation. Phosphorylation is required for TGN localization of the endoprotease. In vivo, exists as di-, mono- and non-phosphorylated forms. |
| Function | Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif. Mediates processing of TGFB1, an essential step in TGF-beta-1 activation. Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells. (Microbial infection) Cleaves and activates diphtheria toxin DT. (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA). (Microbial infection) Cleaves and activates HIV-1 virus Envelope glycoprotein gp160. (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin. (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP. (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells. (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F. |
| Protein Name | FurinDibasic-Processing EnzymePaired Basic Amino Acid Residue-Cleaving EnzymePace |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1442490Reactome: R-HSA-1566948Reactome: R-HSA-1592389Reactome: R-HSA-159782Reactome: R-HSA-167060Reactome: R-HSA-171286Reactome: R-HSA-186797Reactome: R-HSA-1912420Reactome: R-HSA-2173789Reactome: R-HSA-2173796Reactome: R-HSA-5210891Reactome: R-HSA-6809371Reactome: R-HSA-8963889Reactome: R-HSA-9662834Reactome: R-HSA-9679191Reactome: R-HSA-9694614Reactome: R-HSA-9733458Reactome: R-HSA-977225 |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network MembraneSingle-Pass Type I Membrane ProteinCell MembraneSecretedEndosome MembraneShuttles Between The Trans-Golgi Network And The Cell SurfacePropeptide Cleavage Is A Prerequisite For Exit Of Furin Molecules Out Of The Endoplasmic Reticulum (Er)A Second Cleavage Within The Propeptide Occurs In The Trans Golgi Network (Tgn)Followed By The Release Of The Propeptide And The Activation Of Furin |
| Alternative Antibody Names | Anti-Furin antibodyAnti-Dibasic-Processing Enzyme antibodyAnti-Paired Basic Amino Acid Residue-Cleaving Enzyme antibodyAnti-Pace antibodyAnti-FURIN antibodyAnti-FUR antibodyAnti-PACE antibodyAnti-PCSK3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
