• Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Fascin antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 55 kDa
  • Human Hodgkin lymphoma tissue was stained with Anti-Fascin (ABT-FASN) Antibody
  • Human Hodgkin lymphoma tissue was stained with Anti-Fascin (ABT-FASN) Antibody
  • Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Fascin and anti-GAPDH antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1 : Fascin knockout Hela cell lysate Lane 2 : Wide type Hela cell lysatePredicted band size: 55 kDa
  • Human tonsil tissue was stained with Anti-Fascin (ABT-FASN) Antibody
  • Human tonsil tissue was stained with Anti-Fascin (ABT-FASN) Antibody

Anti-FSCN1 antibody (250-350 aa) [ABT-FASN] (STJ196833)

SKU:
STJ196833

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Fascin (250-350 aa) is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: ABT-FASN
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FSCN1
Gene ID: 6624
Uniprot ID: FSCN1_HUMAN
Immunogen Region: 250-350 aa
Specificity: This antibody detects endogenous levels of human Fascin. Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH8.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human Fascin at the amino acid range 250-350
Post Translational Modifications Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.
Function Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.
Protein Name Fascin
55 Kda Actin-Bundling Protein
Singed-Like Protein
P55
Database Links Reactome: R-HSA-6785807
Cellular Localisation Cytoplasm
Cytosol
Cell Cortex
Cytoskeleton
Stress Fiber
Cell Projection
Filopodium
Invadopodium
Microvillus
Cell Junction
Colocalized With Rufy3 And F-Actin At Filipodia Of The Axonal Growth Cone
Colocalized With Dbn1 And F-Actin At The Transitional Domain Of The Axonal Growth Cone
Alternative Antibody Names Anti-Fascin antibody
Anti-55 Kda Actin-Bundling Protein antibody
Anti-Singed-Like Protein antibody
Anti-P55 antibody
Anti-FSCN1 antibody
Anti-FAN1 antibody
Anti-HSN antibody
Anti-SNL antibody

Information sourced from Uniprot.org

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