Anti-Frizzled 2 antibody (Internal) (STJ71475)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-Frizzled 2 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IF/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lys |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | FZD2 |
| Gene ID | 2535 |
| Uniprot ID | FZD2_HUMAN |
| Accession Number | NP_001457.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | RYATLEHPFHCPR |
Additional Info
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium. TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling. |
| Protein Name | Frizzled-2Fz-2Hfz2Fze2 |
| Database Links | Reactome: R-HSA-201681Reactome: R-HSA-373080Reactome: R-HSA-4086398Reactome: R-HSA-4608870Reactome: R-HSA-4641262Reactome: R-HSA-5140745 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Frizzled-2 antibodyAnti-Fz-2 antibodyAnti-Hfz2 antibodyAnti-Fze2 antibodyAnti-FZD2 antibody |
Information sourced from Uniprot.org