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Anti-FN1 antibody (2337-2386 aa) (STJ93098)

Western blot analysis of lysates from HeLa cells, using Fibronectin 1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) 3T3, 2) KB, 3) K562, 4) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using Fibronectin 1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of 3T3 KB K562 293T lysis using FN1 antibody. Antibody was diluted at 1:2000
Western blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1：2000
Western blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1：2000
Western blot analysis of lysates from 1) 3T3, 2) KB, 3) K562, 4) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-FN1 antibody (2337-2386 aa) (STJ93098)

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STJ93098

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Fibronectin (2337-2386 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC-P 1:100-300 ELISA 1:20000 IF 1:100-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	FN1
Gene ID:	2335
Uniprot ID:	FINC_HUMAN
Immunogen Region:	2337-2386 aa
Specificity:	FN1 Polyclonal Antibody detects endogenous levels of FN1 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Fibronectin 1 at the amino acid range 2337-2386

Post Translational Modifications	Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation. Serotonylated on Gln residues by TGM2 in response to hypoxia.
Function	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling.
Protein Name	Fibronectin Fn Cold-Insoluble Globulin Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1474228 Reactome: R-HSA-1474244 Reactome: R-HSA-1566977 Reactome: R-HSA-202733 Reactome: R-HSA-2129379 Reactome: R-HSA-216083 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-354192 Reactome: R-HSA-354194 Reactome: R-HSA-372708 Reactome: R-HSA-381426 Reactome: R-HSA-5674135 Reactome: R-HSA-6785807 Reactome: R-HSA-6802946 Reactome: R-HSA-6802948 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-8874081 Reactome: R-HSA-8957275 Reactome: R-HSA-9634597 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9700645 Reactome: R-HSA-9725370 Reactome: R-HSA-9725371
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Fibronectin antibody Anti-Fn antibody Anti-Cold-Insoluble Globulin antibody Anti-Cig Cleaved Into - Anastellin - Ugl-Y1 - Ugl-Y2 - Ugl-Y3 antibody Anti-FN1 antibody Anti-FN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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