| Host: | Rabbit |
| Applications: | ELISA/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-FLT3 (900-950) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Biotin |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.5-1.5 µg/µl |
| Dilution Range: | WB: 1:2, 500-1:5, 000 ELISA: 1:50, 000 IP: 1:200 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | FLT3 |
| Gene ID: | 2322 |
| Uniprot ID: | FLT3_HUMAN |
| Immunogen Region: | 900-950 |
| Immunogen: | Synthetic peptide taken within amino acid region 900-950 on human Receptor-type tyrosine-protein kinase FLT3 protein. |
| Post Translational Modifications | N-glycosylated, contains complex N-glycans with sialic acid. Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane. Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways. |
| Protein Name | Receptor-Type Tyrosine-Protein Kinase Flt3 Fl Cytokine Receptor Fetal Liver Kinase-2 Flk-2 Fms-Like Tyrosine Kinase 3 Flt-3 Stem Cell Tyrosine Kinase 1 Stk-1 Cd Antigen Cd135 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 Reactome: R-HSA-9607240 Reactome: R-HSA-9645135 Reactome: R-HSA-9702509 Reactome: R-HSA-9702518 Reactome: R-HSA-9702569 Reactome: R-HSA-9702577 Reactome: R-HSA-9702581 Reactome: R-HSA-9702590 Reactome: R-HSA-9702596 Reactome: R-HSA-9702600 Reactome: R-HSA-9702605 Reactome: R-HSA-9702614 Reactome: R-HSA-9702620 Reactome: R-HSA-9702624 Reactome: R-HSA-9702632 Reactome: R-HSA-9702636 Reactome: R-HSA-9702998 Reactome: R-HSA-9703009 Reactome: R-HSA-9703648 Reactome: R-HSA-9706369 Reactome: R-HSA-9706374 Reactome: R-HSA-9706377 |
| Cellular Localisation | Membrane Single-Pass Type I Membrane Protein Endoplasmic Reticulum Lumen Constitutively Activated Mutant Forms With Internal Tandem Duplications Are Less Efficiently Transported To The Cell Surface And A Significant Proportion Is Retained In An Immature Form In The Endoplasmic Reticulum Lumen The Activated Kinase Is Rapidly Targeted For Degradation |
| Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Kinase Flt3 antibody Anti-Fl Cytokine Receptor antibody Anti-Fetal Liver Kinase-2 antibody Anti-Flk-2 antibody Anti-Fms-Like Tyrosine Kinase 3 antibody Anti-Flt-3 antibody Anti-Stem Cell Tyrosine Kinase 1 antibody Anti-Stk-1 antibody Anti-Cd Antigen Cd135 antibody Anti-FLT3 antibody Anti-CD135 antibody Anti-FLK2 antibody Anti-STK1 antibody |
Information sourced from Uniprot.org