• Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 1 Mu g FLI1 antibody (STJ27611). Western blot was performed from the immunoprecipitate using FLI1 antibody (STJ27611) at a dilution of 1:1000.
  • Western blot analysis of various lysates using (STJ27611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FLI1 antibody (1-100) (STJ27611)

SKU:
STJ27611

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FLI1 (1-100) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FLI1
Gene ID: 2313
Uniprot ID: FLI1_HUMAN
Immunogen Region: 1-100
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human FLI1 (NP_002008.2).
Immunogen Sequence: MDGTIKEALSVVSDDQSLFD SAYGAAAHLPKADMTASGSP DYGQPHKINPLPPQQEWINQ PVRVNVKREYDHMNGSRESP VDCSVSKCSKLVGGGESNPM
Function Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-CCAGGAAGT-3'.
Protein Name Friend Leukemia Integration 1 Transcription Factor
Proto-Oncogene Fli-1
Transcription Factor Ergb
Database Links Reactome: R-HSA-9616222
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Friend Leukemia Integration 1 Transcription Factor antibody
Anti-Proto-Oncogene Fli-1 antibody
Anti-Transcription Factor Ergb antibody
Anti-FLI1 antibody

Information sourced from Uniprot.org

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