Anti-FLG antibody (STJ192986)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-Filaggrin for use in IHC and IF in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC-P 1:50-300IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FLG |
| Gene ID | 2312 |
| Uniprot ID | FILA_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | FILA Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate. Undergoes deimination of some arginine residues (citrullination). |
| Function | Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. |
| Protein Name | Filaggrin |
| Database Links | Reactome: R-HSA-6809371Reactome: R-HSA-9725554 |
| Cellular Localisation | Cytoplasmic GranuleIn The Stratum Granulosum Of The EpidermisLocalized Within Keratohyalin GranulesIn Granular Keratinocytes And In Lower CorneocytesColocalizes With Calpain-1/Capn1 |
| Alternative Antibody Names | Anti-Filaggrin antibodyAnti-FLG antibody |
Information sourced from Uniprot.org