| Host: | Goat |
| Applications: | WB |
| Reactivity: | P.pyralis |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal anti-Firefly Luciferase (340aa C-Term) for use in WB in P.pyralis samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: | This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: | 3 mg/mL |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Immunogen Region: | 340aa C-Term |
| Specificity: | In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot. |
| Immunogen: | Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli. |
| Immunogen Sequence: | LTETTSAILITPEGDDKPGA TETTSAILITPEGDDKPGAV ETTSAILITPEGDDKPGAVG TTSAILITPEGDDKPGAVGK TSAILITPEGDDKPGAVGKV SAILITPEGDDKPGAVGKVV AILITPEGDDKPGAVGKVVP ILITPEGDDKPGAVGKVVPF LITPEGDDKPGAVGKVVPFF ITPEGDDKPGAVGKVVPFFE TPEGDDKPGAVGKVVPFFEA |
| Background | Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
Information sourced from Uniprot.org
