| Host: |
Goat |
| Applications: |
WB |
| Reactivity: |
Transfected cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-Firefly Luciferase (340aa C-Term) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Immunogen Region: |
340aa C-Term |
| Specificity: |
In 293HEK cells transfected with cds plasmid detects a band of 62 kDa by Western blot. |
| Immunogen: |
Purified recombinant peptide derived from within residues 340 aa to the C-terminus of Luciferase produced in E. coli. |
| Background | Goat polyclonal antibody to Firefly luciferase. Luciferase is a widely used reporter protein to study gene expression. Luciferase catalyses a bioluminescent reaction which requires luciferin as a substrate, ATP and Mg2+. A cell extract containing luciferase mixed luciferin in the presence of ATP and Mg2+, results in a flash of light that decays rapidly and can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
