Anti-FICD antibody (STJA0009133)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human FICD
STJA0009133
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General Information

Short DescriptionRabbit polyclonal anti-Protein adenylyltransferase FICD for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolFICD
Gene ID11153
Uniprot IDFICD_HUMAN
ImmunogenSynthesized peptide derived from human FICD
SpecificityThis antibody detects endogenous levels of FICD at Human, Mouse, Rat

Additional Info

Post Translational Modifications Auto-AMPylated in vitro.it is unclear whether auto-AMPylation is relevant in vivo. N-glycosylated.predominantly glycosylated at Asn-275.
Function Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.
Protein Name Protein Adenylyltransferase Ficd
Ampylator Ficd
De-Ampylase Ficd
Fic Domain-Containing Protein
Huntingtin Yeast Partner E
Huntingtin-Interacting Protein 13
Hip-13
Huntingtin-Interacting Protein E
Database Links
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Protein Adenylyltransferase Ficd antibody
Anti-Ampylator Ficd antibody
Anti-De-Ampylase Ficd antibody
Anti-Fic Domain-Containing Protein antibody
Anti-Huntingtin Yeast Partner E antibody
Anti-Huntingtin-Interacting Protein 13 antibody
Anti-Hip-13 antibody
Anti-Huntingtin-Interacting Protein E antibody
Anti-FICD antibody
Anti-HIP13 antibody
Anti-HYPE antibody
Anti-UNQ3041 antibody
Anti-PRO9857 antibody

Information sourced from Uniprot.org

Citations

Product Review