• Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain tissue, diluted at 1:2000.

Anti-FH antibody [2B11] (STJ97058)

SKU:
STJ97058

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Fumarate hydratase, mitochondrial is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 2B11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:1000-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FH
Gene ID: 2271
Uniprot ID: FUMH_HUMAN
Specificity: The antibody detects endogenous Flotillin-1 protein.
Immunogen: Recombinant Protein of FH Fumarase
Post Translational Modifications Isoform Cytoplasmic: Phosphorylation at Thr-236 by PRKDC in response to DNA damage promotes translocation to the nucleus and recruitment to DNA double-strand breaks (DSBs).
Function Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable). Isoform Mitochondrial: Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH. Isoform Cytoplasmic: Catalyzes the dehydration of L-malate to fumarate. Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism.fumarate being a by-product of the urea cycle and amino-acid catabolism. Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs): acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2).
Protein Name Fumarate Hydratase - Mitochondrial
Fumarase
Hsfh
Database Links Reactome: R-HSA-71403
Cellular Localisation Isoform Mitochondrial: Mitochondrion
Isoform Cytoplasmic: Cytoplasm
Cytosol
Nucleus
Chromosome
Translocates To The Nucleus In Response To Dna Damage: Localizes To Dna Double-Strand Breaks (Dsbs) Following Phosphorylation By Prkdc
Alternative Antibody Names Anti-Fumarate Hydratase - Mitochondrial antibody
Anti-Fumarase antibody
Anti-Hsfh antibody
Anti-FH antibody

Information sourced from Uniprot.org

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