| Host: | Rabbit |
| Applications: | ELISA/IHC/IP/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-FGFR4 (520-570) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Contains Tris, HCl/Glycine buffer pH 7.4-7.8, 30% Glycerol and 0.5% BSA, along with cryo-protective agents, Hepes, and long-term preservatives (0.02% Sodium Azide). |
| Purification: | Affinity Purified |
| Concentration: | 0.55 µg/µl |
| Dilution Range: | WB: 1:500 ELISA: 1:10, 000 IP: 1:150 IHC: 1:50-1:100 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | FGFR4 |
| Gene ID: | 2264 |
| Uniprot ID: | FGFR4_HUMAN |
| Immunogen Region: | 520-570 |
| Immunogen: | Synthetic peptide taken within amino acid region 520-570 on human Fibroblast growth factor receptor 4 variant protein. |
| Tissue Specificity | Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. |
| Post Translational Modifications | N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19. Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated. Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation.MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling. |
| Protein Name | Fibroblast Growth Factor Receptor 4 Fgfr-4 Cd Antigen Cd334 |
| Database Links | Reactome: R-HSA-109704 Reactome: R-HSA-1257604 Reactome: R-HSA-1307965 Reactome: R-HSA-1839128 Reactome: R-HSA-190322 Reactome: R-HSA-2219530 Reactome: R-HSA-5654228 Reactome: R-HSA-5654712 Reactome: R-HSA-5654719 Reactome: R-HSA-5654720 Reactome: R-HSA-5654733 Reactome: R-HSA-5655291 Reactome: R-HSA-5673001 Reactome: R-HSA-6811558 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Endosome Endoplasmic Reticulum Internalized From The Cell Membrane To Recycling Endosomes And From There Back To The Cell Membrane Isoform 2: Secreted |
| Alternative Antibody Names | Anti-Fibroblast Growth Factor Receptor 4 antibody Anti-Fgfr-4 antibody Anti-Cd Antigen Cd334 antibody Anti-FGFR4 antibody Anti-JTK2 antibody Anti-TKF antibody |
Information sourced from Uniprot.org