• Immunofluorescence analysis of L929 cells using FGD1 Rabbit polyclonal antibody (STJ118802) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of FGD1 in paraffin-embedded mouse kidney using FGD1 Rabbit polyclonal antibody (STJ118802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FGD1 in paraffin-embedded human lung cancer using FGD1 Rabbit polyclonal antibody (STJ118802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of FGD1 in paraffin-embedded rat kidney using FGD1 Rabbit polyclonal antibody (STJ118802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using FGD1 Rabbit polyclonal antibody (STJ118802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-FGD1 antibody (700-800) (STJ118802)

SKU:
STJ118802

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FGD1 (700-800) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FGD1
Gene ID: 2245
Uniprot ID: FGD1_HUMAN
Immunogen Region: 700-800
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 700-800 of human FGD1 (NP_004454.2).
Immunogen Sequence: LLNSTNREDEDTPPNSPNVD LGKRAPTPIREKEVTMCMRC QEPFNSITKRRHHCKACGHV VCGKCSEFRARLVYDNNRSN RVCTDCYVALHGVPGSSPAC S
Tissue Specificity Expressed in fetal heart, brain, lung, kidney and placenta. Less expressed in liver.adult heart, brain, lung, pancreas and skeletal muscle.
Function Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
Protein Name Fyve - Rhogef And Ph Domain-Containing Protein 1
Faciogenital Dysplasia 1 Protein
Rho/Rac Guanine Nucleotide Exchange Factor Fgd1
Rho/Rac Gef
Zinc Finger Fyve Domain-Containing Protein 3
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-9013148
Cellular Localisation Cytoplasm
Cell Projection
Lamellipodium
Ruffle
Cytoskeleton
Associated With Membrane Ruffles And Lamellipodia
Alternative Antibody Names Anti-Fyve - Rhogef And Ph Domain-Containing Protein 1 antibody
Anti-Faciogenital Dysplasia 1 Protein antibody
Anti-Rho/Rac Guanine Nucleotide Exchange Factor Fgd1 antibody
Anti-Rho/Rac Gef antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 3 antibody
Anti-FGD1 antibody
Anti-FGDY antibody
Anti-ZFYVE3 antibody

Information sourced from Uniprot.org

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