Anti-FBXW8 antibody (500-598) (STJ11100092)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal FBXW8 (500-598) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FBXW8 |
| Gene ID | 26259 |
| Uniprot ID | FBXW8_HUMAN |
| Immunogen Region | 500-598 |
| Immunogen Sequence | WDYRMNQKLWEVYSGHPVQH ISFSSHSLITANVPYQTVMR NADLDSFTTHRRHRGLIRAY EFAVDQLAFQSPLPVCRSSC DAMATHYYDLALAFPYNHV |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 500-598 of human FBXW8 (NP_699179.2). |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-85 by mTORC2 promotes FBXW8 stabilization, allowing its translocation to the cytosol in response to insulin. |
| Function | Substrate-recognition component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The Cul7-RING(FBXW8) complex mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. The Cul7-RING(FBXW8) complex also mediates ubiquitination of phosphorylated cyclin-D1 (CCND1). The Cul7-RING(FBXW8) complex is however not a major regulator of CCND1 stability during the G1/S transition. Associated component of the 3M complex, suggesting that it mediates some of 3M complex functions. |
| Protein Name | F-Box/Wd Repeat-Containing Protein 8F-Box And Wd-40 Domain-Containing Protein 8F-Box Only Protein 29 |
| Database Links | Reactome: R-HSA-8951664Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmPerinuclear RegionGolgi ApparatusLocalizes To The Cytosol When Phosphorylated At Ser-85Promoting Irs1 Ubiquitination |
| Alternative Antibody Names | Anti-F-Box/Wd Repeat-Containing Protein 8 antibodyAnti-F-Box And Wd-40 Domain-Containing Protein 8 antibodyAnti-F-Box Only Protein 29 antibodyAnti-FBXW8 antibodyAnti-FBW6 antibodyAnti-FBW8 antibodyAnti-FBX29 antibodyAnti-FBXO29 antibodyAnti-FBXW6 antibody |
Information sourced from Uniprot.org