| Host: | Rabbit |
| Applications: | WB/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Atrogin-1 (N-Term) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000IHC 1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Fbxo32 |
| Gene ID: | 67731 |
| Uniprot ID: | FBX32_MOUSE |
| Immunogen Region: | N-Term |
| Specificity: | This antibody detects a 41 kDa* protein corresponding to the apparent molecular mass of Atrogin on SDS-PAGE immunoblots of mouse muscle tissue lysates. Similar results were seen in C2C12 cell lysate and the band observed in western blot is specifical |
| Immunogen: | A synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of mouse Atrogin. This sequence is highly homologous to human and rat Atrogin (F-box only protein 32). |
| Tissue Specificity | Specifically expressed in cardiac and skeletal muscle. |
| Function | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. |
| Protein Name | F-Box Only Protein 32Atrogin-1Muscle Atrophy F-Box ProteinMafbx |
| Database Links | Reactome: R-MMU-8951664Reactome: -MMU-983168 |
| Cellular Localisation | CytoplasmNucleusShuttles Between Cytoplasm And The Nucleus |
| Alternative Antibody Names | Anti-F-Box Only Protein 32 antibodyAnti-Atrogin-1 antibodyAnti-Muscle Atrophy F-Box Protein antibodyAnti-Mafbx antibodyAnti-Fbxo32 antibody |
Information sourced from Uniprot.org
