• Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle using Fbx32/FBXO32 Rabbit polyclonal antibody (STJ28908) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from RD cells, using Fbx32/FBXO32 Rabbit polyclonal antibody (STJ28908) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of various cell lines, using NTHL1 antibody (A6820) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FBXO32 antibody (206-355) (STJ28908)

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STJ28908

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FBXO32 (206-355) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO32
Gene ID: 114907
Uniprot ID: FBX32_HUMAN
Immunogen Region: 206-355
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 206-355 of human Fbx32/FBOX32 (NP_478136.1).
Immunogen Sequence: WQQQLNNIQITRPAFKGLTF TDLPLCLQLNIMQRLSDGRD LVSLGQAAPDLHVLSEDRLL WKKLCQYHFSERQIRKRLIL SDKGQLDWKKMYFKLVRCYP RKEQYGDTLQLCKHCHILSW KGTDHPCTANNPESCSVSLS PQDFINLFKF
Tissue Specificity Specifically expressed in cardiac and skeletal muscle.
Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
Protein Name F-Box Only Protein 32
Atrogin-1
Muscle Atrophy F-Box Protein
Mafbx
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-9615017
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between Cytoplasm And The Nucleus
Alternative Antibody Names Anti-F-Box Only Protein 32 antibody
Anti-Atrogin-1 antibody
Anti-Muscle Atrophy F-Box Protein antibody
Anti-Mafbx antibody
Anti-FBXO32 antibody

Information sourced from Uniprot.org

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