• Western blot analysis of extracts of various cell lines, using FBXO22 antibody (STJ110665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using FBXO22 antibody (STJ110665) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FBXO22 antibody (104-403) (STJ110665)

SKU:
STJ110665

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FBXO22
Gene ID: 26263
Uniprot ID: FBX22_HUMAN
Immunogen Region: 104-403
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 104-403 of human FBXO22 (NP_671717.1).
Immunogen Sequence: YMADSETFISLEECRGHKRA RKRTSMETALALEKLFPKQC QVLGIVTPGIVVTPMGSGSN RPQEIEIGESGFALLFPQIE GIKIQPFHFIKDPKNLTLER HQLTEVGLLDNPELRVVLVF GYNCCKVGASNYLQQVVSTF SDMNIILAGGQVDNLSSLTS EKNPLDIDASGVVGLSFSGH RIQSATVLLNEDVSDEKTAE AAMQRLKAANIPEHNTIGFM FACVGRGFQYYRAKGNVEA
Tissue Specificity Predominantly expressed in liver, also enriched in cardiac muscle.
Function Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes the proteasome-dependent degradation of key sarcomeric proteins, such as alpha-actinin (ACTN2) and filamin-C (FLNC), essential for maintenance of normal contractile function.
Protein Name F-Box Only Protein 22
F-Box Protein Fbx22p44
Database Links Reactome: R-HSA-8951664
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Z Line
Alternative Antibody Names Anti-F-Box Only Protein 22 antibody
Anti-F-Box Protein Fbx22p44 antibody
Anti-FBXO22 antibody
Anti-FBX22 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance