• Immunohistochemistry analysis of paraffin-embedded rat spleen using Fas Rabbit polyclonal antibody (STJ116183) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Fas antibody (STJ116183) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-FAS antibody (50-150) (STJ116183)

SKU:
STJ116183

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAS (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAS
Gene ID: 355
Uniprot ID: TNR6_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Fas (NP_000034.1).
Immunogen Sequence: EGLHHDGQFCHKPCPPGERK ARDCTVNGDEPDCVPCQEGK EYTDKAHFSSKCRRCRLCDE GHGLEVEINCTRTQNTKCRC KPNFFCNSTVCEHCDPCTKC E
Tissue Specificity Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Post Translational Modifications (Microbial infection) Glycosylated at Arg-250 by enteropathogenic E.coli protein NleB1: arginine GlcNAcylation prevents homotypic/heterotypic death domain interactions. Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 6
Apo-1 Antigen
Apoptosis-Mediating Surface Antigen Fas
Faslg Receptor
Cd Antigen Cd95
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-6803211
Reactome: R-HSA-69416
Reactome: R-HSA-75157
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Isoform 2: Secreted
Isoform 3: Secreted
Isoform 4: Secreted
Isoform 5: Secreted
Isoform 6: Secreted
Alternative Antibody Names Anti-Tumor Necrosis Factor Receptor Superfamily Member 6 antibody
Anti-Apo-1 Antigen antibody
Anti-Apoptosis-Mediating Surface Antigen Fas antibody
Anti-Faslg Receptor antibody
Anti-Cd Antigen Cd95 antibody
Anti-FAS antibody
Anti-APT1 antibody
Anti-FAS1 antibody
Anti-TNFRSF6 antibody

Information sourced from Uniprot.org

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