• Western blot analysis of extracts of various cell lines, using FAM83A antibody (STJ117395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-FAM83A antibody (1-160) (STJ117395)

SKU:
STJ117395

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAM83A (1-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAM83A
Gene ID: 84985
Uniprot ID: FA83A_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human FAM83A (NP_116288.2).
Immunogen Sequence: MSRSRHLGKIRKRLEDVKSQ WVRPARADFSDNESARLATD ALLDGGSEAYWRVLSQEGEV DFLSSVEAQYIQAQAREPPC PPDTLGGAEAGPKGLDSSSL QSGTYFPVASEGSEPALLHS WASAEKPYLKEKSSATVYFQ TVKHNNIRDLVRRCITRTSQ
Post Translational Modifications Phosphorylated upon EGFR activation.
Function Probable proto-oncogene that functions in the epidermal growth factor receptor/EGFR signaling pathway. Activates both RAS/MAPK and PI3K/AKT/TOR signaling cascades downstream of EGFR. Required for the RAS/MAPK signaling cascade activation upon EGFR stimulation, it also activates both signaling cascades independently of EGFR activation.
Protein Name Protein Fam83a
Tumor Antigen Bj-Tsa-9
Tumor-Specific Gene Expressed In Prostate Protein
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Fam83a antibody
Anti-Tumor Antigen Bj-Tsa-9 antibody
Anti-Tumor-Specific Gene Expressed In Prostate Protein antibody
Anti-FAM83A antibody
Anti-TSGP antibody

Information sourced from Uniprot.org

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