• Immunohistochemistry analysis of paraffin-embedded human lung cancer using FAF1 antibody (STJ110089) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using FAF1 antibody (STJ110089) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using FAF1 antibody (STJ110089) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using FAF1 antibody (STJ110089) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-FAF1 antibody (STJ110089)

SKU:
STJ110089

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FAF1 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FAF1
Gene ID: 11124
Uniprot ID: FAF1_HUMAN
Immunogen: A synthetic peptide of human FAF1
Immunogen Sequence: Email for sequence
Tissue Specificity Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.
Function Ubiquitin-binding protein. Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation. Potentiates but cannot initiate FAS-induced apoptosis.
Protein Name Fas-Associated Factor 1
Hfaf1
Ubx Domain-Containing Protein 12
Ubx Domain-Containing Protein 3a
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Fas-Associated Factor 1 antibody
Anti-Hfaf1 antibody
Anti-Ubx Domain-Containing Protein 12 antibody
Anti-Ubx Domain-Containing Protein 3a antibody
Anti-FAF1 antibody
Anti-UBXD12 antibody
Anti-UBXN3A antibody
Anti-CGI-03 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance