• Immunofluorescence analysis of mouse liver cells using Factor IX/ F9 Rabbit polyclonal antibody (STJ23601) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human liver cells using Factor IX/ F9 Rabbit polyclonal antibody (STJ23601) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat liver cells using Factor IX/ F9 Rabbit polyclonal antibody (STJ23601) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using Factor IX/ F9 Rabbit polyclonal antibody (STJ23601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-F9 antibody (29-461) (STJ23601)

SKU:
STJ23601

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Factor IX/F9 (29-461) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: F9
Gene ID: 2158
Uniprot ID: FA9_HUMAN
Immunogen Region: 29-461
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 29-461 of human Factor IX/F9 (NP_000124.1).
Immunogen Sequence: TVFLDHENANKILNRPKRYN SGKLEEFVQGNLERECMEEK CSFEEAREVFENTERTTEFW KQYVDGDQCESNPCLNGGSC KDDINSYECWCPFGFEGKNC ELDVTCNIKNGRCEQFCKNS ADNKVVCSCTEGYRLAENQK SCEPAVPFPCGRVSVSQTSK LTRA
Tissue Specificity Detected in blood plasma (at protein level). Synthesized primarily in the liver and secreted in plasma.
Post Translational Modifications Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Function Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Protein Name Coagulation Factor Ix
Christmas Factor
Plasma Thromboplastin Component
Ptc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain
Database Links Reactome: R-HSA-140834
Reactome: R-HSA-140837
Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Reactome: R-HSA-9672383
Reactome: R-HSA-9672396
Reactome: R-HSA-9673202
Reactome: R-HSA-9673218
Reactome: R-HSA-9673221
Reactome: R-HSA-9673240
Cellular Localisation Secreted
Alternative Antibody Names Anti-Coagulation Factor Ix antibody
Anti-Christmas Factor antibody
Anti-Plasma Thromboplastin Component antibody
Anti-Ptc Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain antibody
Anti-F9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance