Anti-EZH1 antibody (160-280) (STJ28381)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EZH1 (160-280) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EZH1 |
| Gene ID | 2145 |
| Uniprot ID | EZH1_HUMAN |
| Immunogen Region | 160-280 |
| Immunogen Sequence | GEEEMIPGSVLISDAVFLEL VDALNQYSDEEEEGHNDTSD GKQDDSKEDLPVTRKRKRHA IEGNKKSSKKQFPNDMIFSA IASMFPENGVPDDMKERYRE LTEMSDPNALPPQCTPNIDG P |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 160-280 of human EZH1 (NP_001982.2). |
Additional Info
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| Protein Name | Histone-Lysine N-Methyltransferase Ezh1Enx-2Enhancer Of Zeste Homolog 1 |
| Database Links | |
| Cellular Localisation | NucleusColocalizes With Trimethylated 'Lys-27' Of Histone H3 |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Ezh1 antibodyAnti-Enx-2 antibodyAnti-Enhancer Of Zeste Homolog 1 antibodyAnti-EZH1 antibodyAnti-KIAA0388 antibody |
Information sourced from Uniprot.org