Anti-EYA3 antibody (1-300) (STJ110202)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal EYA3 (1-300) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EYA3 |
| Gene ID | 2140 |
| Uniprot ID | EYA3_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MEEEQDLPEQPVKKAKMQES GEQTISQVSNPDVSDQKPET SSLASNLPMSEEIMTCTDYI PRSSNDYTSQMYSAKPYAHI LSVPVSETAYPGQTQYQTLQ QTQPYAVYPQATQTYGLPPF GALWPGMKPESGLIQTPSPS QHSVLTCTTGLTTSQPSPAH YSYPIQASSTNASLISTSST IANIPAAAVASISNQDYPTY TILGQNQYQACYPSSSFGVT GQTNSDAESTTLAATTYQS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2). |
Additional Info
| Post Translational Modifications | Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX. |
| Function | Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye. |
| Protein Name | Protein Phosphatase Eya3Eyes Absent Homolog 3 |
| Database Links | Reactome: R-HSA-5693565 |
| Cellular Localisation | CytoplasmNucleusLocalizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)With Decreasing EfficiencyTranslocalized To The Nucleus By Six2 And Six5And Six4Respectively |
| Alternative Antibody Names | Anti-Protein Phosphatase Eya3 antibodyAnti-Eyes Absent Homolog 3 antibodyAnti-EYA3 antibody |
Information sourced from Uniprot.org