• Western blot analysis of various lysates, using EXT1 Rabbit polyclonal antibody (STJ11105315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EXT1 antibody (300-400) (STJ11105315)

SKU:
STJ11105315

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Exostosin-1 (300-400) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EXT1
Gene ID: 2131
Uniprot ID: EXT1_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human EXT1 (NP_000118.2).
Immunogen Sequence: HGKDWQKHKDSRCDRDNTEY EKYDYREMLHNATFCLVPRG RRLGSFRFLEALQAACVPVM LSNGWELPFSEVINWNQAAV IGDERLLLQIPSTIRSIHQD K
Tissue Specificity Ubiquitous.
Function Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Appears to be a tumor suppressor. Required for the exosomal release of SDCBP, CD63 and syndecan.
Protein Name Exostosin-1
Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase
Multiple Exostoses Protein 1
Putative Tumor Suppressor Protein Ext1
Database Links Reactome: R-HSA-2022928
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Ii Membrane Protein
Golgi Apparatus Membrane
Golgi Apparatus
Cis-Golgi Network Membrane
The Ext1/Ext2 Complex Is Localized In The Golgi Apparatus
Alternative Antibody Names Anti-Exostosin-1 antibody
Anti-Glucuronosyl-N-Acetylglucosaminyl-Proteoglycan/N-Acetylglucosaminyl-Proteoglycan 4-Alpha-N-Acetylglucosaminyltransferase antibody
Anti-Multiple Exostoses Protein 1 antibody
Anti-Putative Tumor Suppressor Protein Ext1 antibody
Anti-EXT1 antibody

Information sourced from Uniprot.org

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