Anti-EXOSC10 antibody (391-441 aa) (STJ194392)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human EXOSX at the amino acid range 391-441
General Information
| Short Description | Rabbit polyclonal anti-Exosome complex component 10 (391-441 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EXOSC10 |
| Gene ID | 5394 |
| Uniprot ID | EXOSX_HUMAN |
| Immunogen | Synthesized peptide derived from the human EXOSX at the amino acid range 391-441 |
| Immunogen Region | 391-441 aa |
| Specificity | This antibody detects endogenous levels of EXOSX at Human/Mouse |
Additional Info
| Function | Catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of MTREX, C1D and MPHOSPH6 with the RNA exosome involved in the maturation of 5.8S rRNA. Plays a role in the recruitment of replication protein A complex (RPA) and RAD51 to DNA double-strand breaks caused by irradiation, contributing to DNA repair by homologous recombination. Regulates levels of damage-induced RNAs in order to prevent DNA-RNA hybrid formation at DNA double-strand breaks and limit DNA end resection after damage. Plays a role in oocyte development, maturation and survival. Required for normal testis development and mitotic division of spermatogonia. Plays a role in proper embryo development. Required for global protein translation. Required for cell proliferation. Regulates metabolism of C9orf72-derived repeat RNA that can be translated into toxic dipeptide repeat proteins. |
| Protein Name | Exosome Complex Component 10Autoantigen Pm/Scl 2P100 Polymyositis-Scleroderma Overlap Syndrome-Associated AutoantigenPolymyositis/Scleroderma Autoantigen 100 KdaPm/Scl-100Polymyositis/Scleroderma Autoantigen 2 |
| Database Links | Reactome: R-HSA-6791226 |
| Cellular Localisation | CytoplasmNucleusNucleolusNucleoplasmStrongly Enriched In The Nucleolus And A Small Amount Has Been Found In Cytoplasm Supporting The Existence Of A Nucleolar Rna Exosome Complex FormArginine-Rich Dipeptide Repeat Proteins Expressed From C9orf72-Derived Repeat Rna Cause Diffuse Nuclear Misdistribution Of Exosc10Relocates To The Dna Double-Strand Breaks In Response To Irradiation |
| Alternative Antibody Names | Anti-Exosome Complex Component 10 antibodyAnti-Autoantigen Pm/Scl 2 antibodyAnti-P100 Polymyositis-Scleroderma Overlap Syndrome-Associated Autoantigen antibodyAnti-Polymyositis/Scleroderma Autoantigen 100 Kda antibodyAnti-Pm/Scl-100 antibodyAnti-Polymyositis/Scleroderma Autoantigen 2 antibodyAnti-EXOSC10 antibodyAnti-PMSCL antibodyAnti-PMSCL2 antibodyAnti-RRP6 antibody |
Information sourced from Uniprot.org