• Biotinylated STJ72290 (0. 03µg/ml) staining of Mouse Liver lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ETFB, Biotinylated antibody (231-243) {Biotin} (STJ73218)

SKU:
STJ73218-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ETFB, Biotinylated (231-243) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.03-0.1µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ETFB
Gene ID: 2109
Uniprot ID: ETFB_HUMAN
Immunogen Region: 231-243
Accession Number: NP_001976.1; NP_001014763.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).
Immunogen Sequence: PQRTAGVKVETTE.
Post Translational Modifications Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from acyl-CoA dehydrogenases.
Function Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.
Protein Name Electron Transfer Flavoprotein Subunit Beta
Beta-Etf
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-8876725
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Electron Transfer Flavoprotein Subunit Beta antibody
Anti-Beta-Etf antibody
Anti-ETFB antibody
Anti-FP585 antibody

Information sourced from Uniprot.org

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