Anti-ESR2 antibody (1-153) (STJ23576)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Estrogen Receptor beta (1-153) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ESR2 |
| Gene ID | 2100 |
| Uniprot ID | ESR2_HUMAN |
| Immunogen Region | 1-153 |
| Immunogen Sequence | MDIKNSPSSLNSPSSYNCSQ SILPLEHGSIYIPSSYVDSH HEYPAMTFYSPAVMNYSIPS NVTNLEGGPGRQTTSPNVLW PTPGHLSPLVVHRQLSHLYA EPQKSPWCEARSLEHTLPVN RETLKRKVSGNRCASPVTGP GSKRDAHFCAVCS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human Estrogen Receptor beta (NP_001428.1). |
Additional Info
| Tissue Specificity | Isoform 1: Expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2: Expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4: Expressed in the testis. Isoform 5: Expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6: Expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. |
| Post Translational Modifications | Phosphorylation at Ser-87 and Ser-105 recruits NCOA1. |
| Function | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform 2: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. |
| Protein Name | Estrogen Receptor BetaEr-BetaNuclear Receptor Subfamily 3 Group A Member 2 |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-383280Reactome: R-HSA-6811558Reactome: R-HSA-8939211Reactome: R-HSA-9009391 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Estrogen Receptor Beta antibodyAnti-Er-Beta antibodyAnti-Nuclear Receptor Subfamily 3 Group A Member 2 antibodyAnti-ESR2 antibodyAnti-ESTRB antibodyAnti-NR3A2 antibody |
Information sourced from Uniprot.org