Anti-ESPL1 antibody (1220-1500) (STJ117561)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ESPL1 (1220-1500) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ESPL1 |
| Gene ID | 9700 |
| Uniprot ID | ESPL1_HUMAN |
| Immunogen Region | 1220-1500 |
| Immunogen Sequence | DEILAQAYTLLALEGLNQPS NESLQKVLQSGLKFVAARIP HLEPWRASLLLIWALTKLGG LSCCTTQLFASSWGWQPPLI KSVPGSEPSKTQGQKRSGRG RQKLASAPLRLNNTSQKGLE GRGLPCTPKPPDRIRQAGPH VPFTVFEEVCPTESKPEVPQ APRVQQRVQTRLKVNFSDDS DLEDPVSAEAWLAEEPKRRG TASRGRGRARKGLSLKTDAV VAPGSAPGNPGLNGRSRRA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1220-1500 of human ESPL1 (NP_036423.4). |
Additional Info
| Post Translational Modifications | Autocleaves. This function, which is not essential for its protease activity, is unknown. Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation. |
| Function | Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. |
| Protein Name | SeparinCaspase-Like Protein Espl1Extra Spindle Poles-Like 1 ProteinSeparase |
| Database Links | Reactome: R-HSA-2467813 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Separin antibodyAnti-Caspase-Like Protein Espl1 antibodyAnti-Extra Spindle Poles-Like 1 Protein antibodyAnti-Separase antibodyAnti-ESPL1 antibodyAnti-ESP1 antibodyAnti-KIAA0165 antibody |
Information sourced from Uniprot.org