| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ESPL1 |
| Gene ID: | 9700 |
| Uniprot ID: | ESPL1_HUMAN |
| Immunogen Region: | 1220-1500 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1220-1500 of human ESPL1 (NP_036423.4). |
| Immunogen Sequence: | DEILAQAYTLLALEGLNQPS NESLQKVLQSGLKFVAARIP HLEPWRASLLLIWALTKLGG LSCCTTQLFASSWGWQPPLI KSVPGSEPSKTQGQKRSGRG RQKLASAPLRLNNTSQKGLE GRGLPCTPKPPDRIRQAGPH VPFTVFEEVCPTESKPEVPQ APRVQQRVQTRLKVNFSDDS DLEDPVSAEAWLAEEPKRRG TASRGRGRARKGLSLKTDAV VAPGSAPGNPGLNGRSRRA |
| Post Translational Modifications | Autocleaves. This function, which is not essential for its protease activity, is unknown. Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation. |
| Function | Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. |
| Protein Name | SeparinCaspase-Like Protein Espl1Extra Spindle Poles-Like 1 ProteinSeparase |
| Database Links | Reactome: R-HSA-2467813 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Separin antibodyAnti-Caspase-Like Protein Espl1 antibodyAnti-Extra Spindle Poles-Like 1 Protein antibodyAnti-Separase antibodyAnti-ESPL1 antibodyAnti-ESP1 antibodyAnti-KIAA0165 antibody |
Information sourced from Uniprot.org