Anti-ERV3 antibody (Internal) (STJ72419)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ERV3 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:4000.WB: This product has been successfully used in WB on Human: Nahas MR et al, Br J Haematol. 2019 May;185 (4) :679-690. PMID: 30828801.IHC: Paraffin embedded Human Placenta. Recommended |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ERV3-1 |
| Gene ID | 2086 |
| Uniprot ID | ENR1_HUMAN |
| Accession Number | NP_001007254.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | PQDNFTLTASS |
Additional Info
| Post Translational Modifications | Specific enzymatic cleavages in vivo yield the mature SU and TM proteins. Has been mainly detected in vivo as an 65 kDa unprocessed polyprotein precursor. The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion. |
| Function | Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its fusogenic properties. It can inhibit cell growth through decrease expression of cyclin B1 and increased expression of p21 in vitro. SU mediates receptor recognition. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane. |
| Protein Name | Endogenous Retrovirus Group 3 Member 1 Env PolyproteinErv-3 Envelope ProteinErv3 Envelope ProteinErv3-1 Envelope ProteinEnvelope PolyproteinHerv-R Envelope ProteinErv-R Envelope ProteinHerv-R_7q21.2 Provirus Ancestral Env Polyprotein Cleaved Into - Surface ProteinSu - Transmembrane ProteinTm |
| Cellular Localisation | Virion |
| Alternative Antibody Names | Anti-Endogenous Retrovirus Group 3 Member 1 Env Polyprotein antibodyAnti-Erv-3 Envelope Protein antibodyAnti-Erv3 Envelope Protein antibodyAnti-Erv3-1 Envelope Protein antibodyAnti-Envelope Polyprotein antibodyAnti-Herv-R Envelope Protein antibodyAnti-Erv-R Envelope Protein antibodyAnti-Herv-R_7q21.2 Provirus Ancestral Env Polyprotein Cleaved Into - Surface Protein antibodyAnti-Su - Transmembrane Protein antibodyAnti-Tm antibodyAnti-ERV3-1 antibodyAnti-ERV3 antibody |
Information sourced from Uniprot.org