• Western blot analysis of extracts of various cell lines, using ERp29 antibody (STJ110266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using ERp29 Rabbit polyclonal antibody (STJ110266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts from normal (control) and ERp29 knockout (KO) 293T cells, using ERp29 antibody (STJ110266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ERP29 antibody (33-261) (STJ110266)

SKU:
STJ110266

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ERP29 (33-261) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERP29
Gene ID: 10961
Uniprot ID: ERP29_HUMAN
Immunogen Region: 33-261
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 33-261 of human ERp29 (NP_006808.1).
Immunogen Sequence: LHTKGALPLDTVTFYKVIPK SKFVLVKFDTQYPYGEKQDE FKRLAENSASSDDLLVAEVG ISDYGDKLNMELSEKYKLDK ESYPVFYLFRDGDFENPVPY TGAVKVGAIQRWLKGQGVYL GMPGCLPVYDALAGEFIRAS GVEARQALLKQGQDNLSSVK ETQKKWAEQYLKIMGKILDQ GEDFPASEMTRIARLIEKNK MSDGKKEELQKSLNILTAFQ KKGAEKEEL
Tissue Specificity Ubiquitous. Mostly expressed in secretory tissues.
Function Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER.
Protein Name Endoplasmic Reticulum Resident Protein 29
Erp29
Endoplasmic Reticulum Resident Protein 28
Erp28
Endoplasmic Reticulum Resident Protein 31
Erp31
Cellular Localisation Endoplasmic Reticulum Lumen
Melanosome
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Endoplasmic Reticulum Resident Protein 29 antibody
Anti-Erp29 antibody
Anti-Endoplasmic Reticulum Resident Protein 28 antibody
Anti-Erp28 antibody
Anti-Endoplasmic Reticulum Resident Protein 31 antibody
Anti-Erp31 antibody
Anti-ERP29 antibody
Anti-C12orf8 antibody
Anti-ERP28 antibody

Information sourced from Uniprot.org

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