Anti-ERK5 antibody [R07-9G1] (STJA0036146)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human ERK5
General Information
| Short Description | Rabbit monoclonal anti-ERK5 for use in WB, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R07-9G1 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MAPK7 |
| Gene ID | 5598 |
| Uniprot ID | MK07_HUMAN |
| Immunogen | A synthesized peptide derived from human ERK5 |
Additional Info
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. |
| Post Translational Modifications | Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. |
| Function | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction. |
| Protein Name | Mitogen-Activated Protein Kinase 7Map Kinase 7Mapk 7Big Map Kinase 1Bmk-1Extracellular Signal-Regulated Kinase 5Erk-5 |
| Database Links | Reactome: R-HSA-198753Reactome: R-HSA-198765Reactome: R-HSA-202670Reactome: R-HSA-2559582Reactome: R-HSA-881907Reactome: R-HSA-8853659 |
| Cellular Localisation | CytoplasmNucleusPml BodyTranslocates To The Nucleus Upon Activation |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 7 antibodyAnti-Map Kinase 7 antibodyAnti-Mapk 7 antibodyAnti-Big Map Kinase 1 antibodyAnti-Bmk-1 antibodyAnti-Extracellular Signal-Regulated Kinase 5 antibodyAnti-Erk-5 antibodyAnti-MAPK7 antibodyAnti-BMK1 antibodyAnti-ERK5 antibodyAnti-PRKM7 antibody |
Information sourced from Uniprot.org