Anti-ERCC6 antibody (141-190 aa) (STJ92493)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human ERCC6 at the amino acid range 141-190
General Information
| Short Description | Rabbit polyclonal anti-DNA excision repair protein ERCC-6 (141-190 aa) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ERCC6 |
| Gene ID | 2074 |
| Uniprot ID | ERCC6_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human ERCC6 at the amino acid range 141-190 |
| Immunogen Region | 141-190 aa |
| Specificity | CSB Polyclonal Antibody detects endogenous levels of CSB protein. |
Additional Info
| Function | Essential factor involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes. Plays a central role in the initiation of the TC-NER process: specifically recognizes and binds RNA polymerase II stalled at a lesion, and mediates recruitment of ERCC8/CSA, initiating DNA damage excision by TFIIH recruitment. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. Acts as a chromatin remodeler at DSBs.DNA-dependent ATPase-dependent activity is essential for this function. Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation.DNA-dependent ATPase activity is essential for this function. Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle. Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing premature entry of cells into mitosis following the induction of DNA DSBs. Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR. Required for stable recruitment of ELOA and CUL5 to DNA damage sites. Also involved in UV-induced translocation of ERCC8 to the nuclear matrix. Essential for neuronal differentiation and neuritogenesis.regulates transcription and chromatin remodeling activities required during neurogenesis. |
| Protein Name | Dna Excision Repair Protein Ercc-6Atp-Dependent Helicase Ercc6Cockayne Syndrome Protein Csb |
| Database Links | Reactome: R-HSA-427389Reactome: R-HSA-5250924Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-73762 |
| Cellular Localisation | NucleusChromosomeRecognizes And Binds Rna Polymerase Ii Stalled At Dna Damage Sites |
| Alternative Antibody Names | Anti-Dna Excision Repair Protein Ercc-6 antibodyAnti-Atp-Dependent Helicase Ercc6 antibodyAnti-Cockayne Syndrome Protein Csb antibodyAnti-ERCC6 antibodyAnti-CSB antibody |
Information sourced from Uniprot.org