• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from COLO cells, using ERCC5 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using XPG Polyclonal Antibody diluted at 1：2000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ERCC5 antibody (131-180 aa) (STJ96280)

SKU:
STJ96280

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA excision repair protein ERCC-5 (131-180 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERCC5
Gene ID: 2073
Uniprot ID: ERCC5_HUMAN
Immunogen Region: 131-180 aa
Specificity: XPG Polyclonal Antibody detects endogenous levels of XPG protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ERCC5 at the amino acid range 131-180
Protein Name Dna Excision Repair Protein Ercc-5
Dna Repair Protein Complementing Xp-G Cells
Xpg
Xeroderma Pigmentosum Group G-Complementing Protein
Database Links Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Cellular Localisation Nucleus
Chromosome
Colocalizes With Rad51 To Nuclear Foci In S Phase
Localizes To Dna Double-Strand Breaks (Dbs) During Replication Stress
Colocalizes With Brca2 To Nuclear Foci Following Dna Replication Stress
Alternative Antibody Names Anti-Dna Excision Repair Protein Ercc-5 antibody
Anti-Dna Repair Protein Complementing Xp-G Cells antibody
Anti-Xpg antibody
Anti-Xeroderma Pigmentosum Group G-Complementing Protein antibody
Anti-ERCC5 antibody
Anti-ERCM2 antibody
Anti-XPG antibody
Anti-XPGC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance