• Western blot analysis using TFIIH p89 monoclonal antibody against PC12, HepG2 cell lysate.

Anti-ERCC3 antibody (C-Term) (STJ98562)

SKU:
STJ98562

Current Stock:
Host: Mouse
Applications: WB
Reactivity: Human/Mouse/Rat/Cow/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-General transcription and DNA repair factor IIH helicase subunit XPB (C-Term) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol.
Purification: Affinity purification
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ERCC3
Gene ID: 2071
Uniprot ID: ERCC3_HUMAN
Immunogen Region: C-Term
Specificity: TFIIH p89 Monoclonal Antibody detects endogenous levels of TFIIH p89 protein.
Immunogen: Purified recombinant human TFIIH p89 (C-terminus) protein fragments expressed in E.coli.
Function ATP-dependent 3'-5' DNA helicase/translocase. Binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening.it may wrap around the damaged DNA wedging it open, causing localized melting that allows XPD/ERCC2 helicase to anchor. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. In transcription pre-initiation complexes induces and propagates a DNA twist to open DNA. Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex.large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA. XPA retains XPB/ERCC3 at the 5' end of a DNA bubble (mimicking DNA damage).
Protein Name General Transcription And Dna Repair Factor Iih Helicase/Translocase Subunit Xpb
Tfiih Subunit Xpb
Basic Transcription Factor 2 89 Kda Subunit
Btf2 P89
Dna 3'-5' Helicase/Translocase Xpb
Dna Excision Repair Protein Ercc-3
Dna Repair Protein Complementing Xp-B Cells
Tfiih Basal Transcription Factor Complex 89 Kda Subunit
Tfiih 89 Kda Subunit
Tfiih P89
Xeroderma Pigmentosum Group B-Complementing Protein
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-167200
Reactome: R-HSA-167246
Reactome: R-HSA-427413
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-674695
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-6796648
Reactome: R-HSA-72086
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-73863
Reactome: R-HSA-75953
Reactome: R-HSA-75955
Reactome: R-HSA-76042
Reactome: R-HSA-77075
Cellular Localisation Nucleus
Alternative Antibody Names Anti-General Transcription And Dna Repair Factor Iih Helicase/Translocase Subunit Xpb antibody
Anti-Tfiih Subunit Xpb antibody
Anti-Basic Transcription Factor 2 89 Kda Subunit antibody
Anti-Btf2 P89 antibody
Anti-Dna 3'-5' Helicase/Translocase Xpb antibody
Anti-Dna Excision Repair Protein Ercc-3 antibody
Anti-Dna Repair Protein Complementing Xp-B Cells antibody
Anti-Tfiih Basal Transcription Factor Complex 89 Kda Subunit antibody
Anti-Tfiih 89 Kda Subunit antibody
Anti-Tfiih P89 antibody
Anti-Xeroderma Pigmentosum Group B-Complementing Protein antibody
Anti-ERCC3 antibody
Anti-XPB antibody
Anti-XPBC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance