| Host: | Rabbit |
| Applications: | WB/IF |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-ERCC2 (1-300) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ERCC2 |
| Gene ID: | 2068 |
| Uniprot ID: | ERCC2_HUMAN |
| Immunogen Region: | 1-300 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human XPD/XPD/ERCC2 (NP_001124339.1). |
| Immunogen Sequence: | MRELKRTLDAKGHGVLEMPS GTGKTVSLLALIMAYQRAYP LEVTKLIYCSRTVPEIEKVI EELRKLLNFYEKQEGEKLPF LGLALSSRKNLCIHPEVTPL RFGKDVDGKCHSLTASYVRA QYQHDTSLPHCRFYEEFDAH GREVPLPAGIYNLDDLKALG RRQGWCPYFLARYSILHANV VVYSYHYLLDPKIADLVSKE LARKAVVVFDEAHNIDNVCI DSMSVNLTRRTLDRCQGNL |
| Post Translational Modifications | ISGylated. |
| Function | ATP-dependent 5'-3' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase Subunit XpdTfiih Subunit XpdBasic Transcription Factor 2 80 Kda SubunitBtf2 P80CxpdDna Excision Repair Protein Ercc-2Dna Repair Protein Complementing Xp-D CellsTfiih Basal Transcription Factor Complex 80 Kda SubunitTfiih 80 Kda SubunitTfiih P80Xeroderma Pigmentosum Group D-Complementing Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167246Reactome: R-HSA-2564830Reactome: R-HSA-427413Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-72086Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73863Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonSpindle |
| Alternative Antibody Names | Anti-General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd antibodyAnti-Tfiih Subunit Xpd antibodyAnti-Basic Transcription Factor 2 80 Kda Subunit antibodyAnti-Btf2 P80 antibodyAnti-Cxpd antibodyAnti-Dna Excision Repair Protein Ercc-2 antibodyAnti-Dna Repair Protein Complementing Xp-D Cells antibodyAnti-Tfiih Basal Transcription Factor Complex 80 Kda Subunit antibodyAnti-Tfiih 80 Kda Subunit antibodyAnti-Tfiih P80 antibodyAnti-Xeroderma Pigmentosum Group D-Complementing Protein antibodyAnti-ERCC2 antibodyAnti-XPD antibodyAnti-XPDC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
