| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ERCC2 |
| Gene ID: | 2068 |
| Uniprot ID: | ERCC2_HUMAN |
| Immunogen Region: | 1-180 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human XPD/XPD/ERCC2 (NP_001124339.1). |
| Immunogen Sequence: | MRELKRTLDAKGHGVLEMPS GTGKTVSLLALIMAYQRAYP LEVTKLIYCSRTVPEIEKVI EELRKLLNFYEKQEGEKLPF LGLALSSRKNLCIHPEVTPL RFGKDVDGKCHSLTASYVRA QYQHDTSLPHCRFYEEFDAH GREVPLPAGIYNLDDLKALG RRQGWCPYFLARYSILHANV |
| Post Translational Modifications | ISGylated. |
| Function | ATP-dependent 5'-3' DNA helicase. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, not absolutely essential for minimal transcription in vitro. Required for transcription-coupled nucleotide excision repair (NER) of damaged DNA.recognizes damaged bases. Sequestered in chromatin on UV-damaged DNA. When complexed to CDK-activating kinase (CAK), involved in transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATP-dependent helicase activity of XPD/ERCC2 is required for DNA opening. Involved in DNA lesion verification. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. XPD/ERCC2 acts by forming a bridge between CAK and the core-TFIIH complex. The structure of the TFIIH transcription complex differs from the NER-TFIIH complex.large movements by XPD/ERCC2 and XPB/ERCC3 are stabilized by XPA which allow this subunit to contact ssDNA. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. |
| Protein Name | General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd Tfiih Subunit Xpd Basic Transcription Factor 2 80 Kda Subunit Btf2 P80 Cxpd Dna 5'-3' Helicase Xpd Dna Excision Repair Protein Ercc-2 Dna Repair Protein Complementing Xp-D Cells Tfiih Basal Transcription Factor Complex 80 Kda Subunit Tfiih 80 Kda Subunit Tfiih P80 Xeroderma Pigmentosum Group D-Complementing Protein |
| Database Links | Reactome: R-HSA-112382 Reactome: R-HSA-113418 Reactome: R-HSA-167152 Reactome: R-HSA-167158 Reactome: R-HSA-167160 Reactome: R-HSA-167161 Reactome: R-HSA-167162 Reactome: R-HSA-167172 Reactome: R-HSA-167200 Reactome: R-HSA-167246 Reactome: R-HSA-2564830 Reactome: R-HSA-427413 Reactome: R-HSA-5696395 Reactome: R-HSA-5696400 Reactome: R-HSA-674695 Reactome: R-HSA-6781823 Reactome: R-HSA-6781827 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6796648 Reactome: R-HSA-72086 Reactome: R-HSA-73762 Reactome: R-HSA-73772 Reactome: R-HSA-73776 Reactome: R-HSA-73779 Reactome: R-HSA-73863 Reactome: R-HSA-75953 Reactome: R-HSA-75955 Reactome: R-HSA-76042 Reactome: R-HSA-77075 |
| Cellular Localisation | Nucleus Cytoplasm Cytoskeleton Spindle |
| Alternative Antibody Names | Anti-General Transcription And Dna Repair Factor Iih Helicase Subunit Xpd antibody Anti-Tfiih Subunit Xpd antibody Anti-Basic Transcription Factor 2 80 Kda Subunit antibody Anti-Btf2 P80 antibody Anti-Cxpd antibody Anti-Dna 5'-3' Helicase Xpd antibody Anti-Dna Excision Repair Protein Ercc-2 antibody Anti-Dna Repair Protein Complementing Xp-D Cells antibody Anti-Tfiih Basal Transcription Factor Complex 80 Kda Subunit antibody Anti-Tfiih 80 Kda Subunit antibody Anti-Tfiih P80 antibody Anti-Xeroderma Pigmentosum Group D-Complementing Protein antibody Anti-ERCC2 antibody Anti-XPD antibody Anti-XPDC antibody |
Information sourced from Uniprot.org