Anti-ERCC1 antibody [7F6-2A10-E8] (STJA0032467)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2b
ImmunogenPurified recombinant human ERCC1 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-ERCC1 for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 7F6-2A10-E8 |
| Isotype | IgG2b |
| Conjugation | Unconjugated |
| Concentration | 18 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | ERCC1 |
| Gene ID | 2067 |
| Uniprot ID | ERCC1_HUMAN |
| Immunogen | Purified recombinant human ERCC1 protein fragments expressed in E.coli. |
Additional Info
| Post Translational Modifications | Ubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Deubiquitinated by USP45. |
| Function | Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4. Isoform 2: Not functional in the nucleotide excision repair pathway. Isoform 3: Not functional in the nucleotide excision repair pathway. Isoform 4: Not functional in the nucleotide excision repair pathway. |
| Protein Name | Dna Excision Repair Protein Ercc-1 |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6783310 |
| Cellular Localisation | Isoform 1: NucleusIsoform 2: CytoplasmNucleusIsoform 3: NucleusIsoform 4: Nucleus |
| Alternative Antibody Names | Anti-Dna Excision Repair Protein Ercc-1 antibodyAnti-ERCC1 antibody |
Information sourced from Uniprot.org