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Anti-ERBB2 antibody [11H9] (STJ96947)

Western blot analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
IHC staining of human breast cancer tissue, diluted at 1:200.
Western blot analysis of lysates from 1) Hela, 2) A431, 3) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Immunofluorescence analysis of Rat-spleen tissue. 1, HER2 monoclonal antibody (11H9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) Mouse Brain, diluted at 1:4000.
Immunofluorescence analysis of Mouse-spleen tissue. 1, HER2 monoclonal antibody (11H9) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, HER2 monoclonal antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, HER2 monoclonal antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, HER2 monoclonal antibody (11H9) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ERBB2 antibody [11H9] (STJ96947)

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STJ96947

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IF/IHC
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Receptor tyrosine-protein kinase erbB-2 is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	11H9
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range:	WB 1:2000-4000 IHC 1:200 IF 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	ERBB2
Gene ID:	2064
Uniprot ID:	ERBB2_HUMAN
Specificity:	The antibody detects endogenous ErbB-2/HER-2 proteins.
Immunogen:	Synthetic Peptide of HER2

Post Translational Modifications	Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12.
Function	Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Protein Name	Receptor Tyrosine-Protein Kinase Erbb-2 Metastatic Lymph Node Gene 19 Protein Mln 19 Proto-Oncogene Neu Proto-Oncogene C-Erbb-2 Tyrosine Kinase-Type Cell Surface Receptor Her2 P185erbb2 Cd Antigen Cd340
Database Links	Reactome: R-HSA-1227986 Reactome: R-HSA-1250196 Reactome: R-HSA-1251932 Reactome: R-HSA-1257604 Reactome: R-HSA-1306955 Reactome: R-HSA-1358803 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Reactome: R-HSA-2219530 Reactome: R-HSA-416572 Reactome: R-HSA-5673001 Reactome: R-HSA-6785631 Reactome: R-HSA-6811558 Reactome: R-HSA-8847993 Reactome: R-HSA-8863795 Reactome: R-HSA-8866910 Reactome: R-HSA-9634285 Reactome: R-HSA-9652282 Reactome: R-HSA-9664565 Reactome: R-HSA-9665233 Reactome: R-HSA-9665244 Reactome: R-HSA-9665245 Reactome: R-HSA-9665246 Reactome: R-HSA-9665247 Reactome: R-HSA-9665249 Reactome: R-HSA-9665250 Reactome: R-HSA-9665251 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686 Reactome: R-HSA-9665737
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Cell Projection Ruffle Membrane Internalized From The Cell Membrane In Response To Egf Stimulation Isoform 1: Cell Membrane Early Endosome Cytoplasm Perinuclear Region Nucleus Translocation To The Nucleus Requires Endocytosis Probably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1 Also Detected In Vps35-Positive Endosome-To-Tgn Retrograde Vesicles Isoform 2: Cytoplasm Isoform 3: Cytoplasm
Alternative Antibody Names	Anti-Receptor Tyrosine-Protein Kinase Erbb-2 antibody Anti-Metastatic Lymph Node Gene 19 Protein antibody Anti-Mln 19 antibody Anti-Proto-Oncogene Neu antibody Anti-Proto-Oncogene C-Erbb-2 antibody Anti-Tyrosine Kinase-Type Cell Surface Receptor Her2 antibody Anti-P185erbb2 antibody Anti-Cd Antigen Cd340 antibody Anti-ERBB2 antibody Anti-HER2 antibody Anti-MLN19 antibody Anti-NEU antibody Anti-NGL antibody

Information sourced from Uniprot.org

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