Anti-EPRS1 antibody (STJ193060)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-Bifunctional glutamate/proline--tRNA ligase for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EPRS1 |
| Gene ID | 2058 |
| Uniprot ID | SYEP_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | SYEP Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). Upon interferon-gamma stimulation, EPRS1 undergoes phosphorylation, causing its dissociation from the aminoacyl-tRNA synthetase multienzyme complex. It is recruited to form the GAIT complex, which binds to stem loop-containing GAIT elements found in the 3'-UTR of various inflammatory mRNAs, such as ceruloplasmin. The GAIT complex inhibits the translation of these mRNAs, allowing interferon-gamma to redirect the function of EPRS1 from protein synthesis to translation inhibition in specific cell contexts. Furthermore, it can function as a downstream effector in the mTORC1 signaling pathway, by promoting the translocation of SLC27A1 from the cytoplasm to the plasma membrane where it mediates the uptake of long-chain fatty acid by adipocytes. Thereby, EPRS1 also plays a role in fat metabolism and more indirectly influences lifespan. |
| Protein Name | Bifunctional Glutamate/Proline--Trna LigaseBifunctional Aminoacyl-Trna SynthetaseCell Proliferation-Inducing Gene 32 ProteinGlutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna LigaseGlutamyl-Trna SynthetaseGlurs - Proline--Trna LigaseProlyl-Trna Synthetase |
| Database Links | Reactome: R-HSA-2408522Reactome: R-HSA-379716Reactome: R-HSA-6782315Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmCytosolMembranePeripheral Membrane ProteinTranslocates From Cytosol To Membranes Upon Phosphorylation At Ser-999 |
| Alternative Antibody Names | Anti-Bifunctional Glutamate/Proline--Trna Ligase antibodyAnti-Bifunctional Aminoacyl-Trna Synthetase antibodyAnti-Cell Proliferation-Inducing Gene 32 Protein antibodyAnti-Glutamatyl-Prolyl-Trna Synthetase Includes - Glutamate--Trna Ligase antibodyAnti-Glutamyl-Trna Synthetase antibodyAnti-Glurs - Proline--Trna Ligase antibodyAnti-Prolyl-Trna Synthetase antibodyAnti-EPRS1 antibodyAnti-EPRS antibodyAnti-GLNS antibodyAnti-PARS antibodyAnti-QARS antibodyAnti-QPRS antibodyAnti-PIG32 antibody |
Information sourced from Uniprot.org