• Immunohistochemistry analysis of EPN2 in paraffin-embedded Human colon carcinoma using EPN2 Rabbit polyclonal antibody (STJ118546) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using EPN2 Rabbit polyclonal antibody (STJ118546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-EPN2 antibody (120-300) (STJ118546)

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STJ118546

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EPN2 (120-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EPN2
Gene ID: 22905
Uniprot ID: EPN2_HUMAN
Immunogen Region: 120-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-300 of human EPN2 (NP_683723.2).
Immunogen Sequence: GINVREKSKQLVALLKDEER LKAERAQALKTKERMAQVAT GMGSNQITFGRGSSQPNLST SHSEQEYGKAGGSPASYHGS TSPRVSSELEQARPQTSGEE ELQLQLALAMSREVAEQEER LRRGDDLRLQMALEESRRDT VKIPKKKEHGSLPQQTTLLD LMDALPSSGPAAQKAEPWGP S
Tissue Specificity Highest expression is found in brain. Detected at lower levels in lung and liver.
Post Translational Modifications Ubiquitinated.
Function Plays a role in the formation of clathrin-coated invaginations and endocytosis.
Protein Name Epsin-2
Eps-15-Interacting Protein 2
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Clathrin-Coated Vesicle
In Punctate Structures Throughout The Cell
Associated With Clathrin-Coated Vesicles
And Particularly Concentrated In The Region Of The Golgi Complex
Alternative Antibody Names Anti-Epsin-2 antibody
Anti-Eps-15-Interacting Protein 2 antibody
Anti-EPN2 antibody
Anti-KIAA1065 antibody

Information sourced from Uniprot.org

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