| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-EPHA5 (1-130) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
EPHA5 |
| Gene ID: |
2044 |
| Uniprot ID: |
EPHA5_HUMAN |
| Immunogen Region: |
1-130 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human EPHA5 (NP_004430.4). |
| Immunogen Sequence: |
MRGSGPRGAGRRRPPSGGGD TPITPASLAGCYSAPRRAPL WTCLLLCAALRTLLASPSNE VNLLDSRTVMGDLGWIAFPK NGWEEIGEVDENYAPIHTYQ VCKVMEQNQNNWLLTSWISN EGASRIFIEL |
| Tissue Specificity | Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord. |
| Post Translational Modifications | Phosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion. |
| Function | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. |
| Protein Name | Ephrin Type-A Receptor 5Brain-Specific KinaseEph Homology Kinase 1Ehk-1Eph-Like Kinase 7Ek7Hek7 |
| Database Links | Reactome: R-HSA-2682334Reactome: R-HSA-3928663Reactome: R-HSA-3928665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonDendrite |
| Alternative Antibody Names | Anti-Ephrin Type-A Receptor 5 antibodyAnti-Brain-Specific Kinase antibodyAnti-Eph Homology Kinase 1 antibodyAnti-Ehk-1 antibodyAnti-Eph-Like Kinase 7 antibodyAnti-Ek7 antibodyAnti-Hek7 antibodyAnti-EPHA5 antibodyAnti-BSK antibodyAnti-EHK1 antibodyAnti-HEK7 antibodyAnti-TYRO4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
