| Host: | Rabbit |
| Applications: | IF/WB/IHC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Histone acetyltransferase p300 (Internal) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IF 1:50-200WB 1:500-1:2000IHC-P 1:100-1:300ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | EP300 |
| Gene ID: | 2033 |
| Uniprot ID: | EP300_HUMAN |
| Immunogen Region: | Internal |
| Specificity: | p300 Polyclonal Antibody detects endogenous levels of p300 protein. |
| Immunogen: | Synthesized peptide derived from the Internal region of human p300. |
| Function | Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for non-histone targets, such as ALX1, HDAC1, PRMT1 or SIRT2. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of p53/TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Following DNA damage, forms a stress-responsive p53/TP53 coactivator complex with JMY which mediates p53/TP53 acetylation, thereby increasing p53/TP53-dependent transcription and apoptosis. Promotes chromatin acetylation in heat shock responsive HSP genes during the heat shock response (HSR), thereby stimulating HSR transcription. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acetylates 'Lys-247' of EGR2. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription.exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity. Acetylates XBP1 isoform 2.acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity. Acetylates PCNA.acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER). Acetylates MEF2D. Acetylates and stabilizes ZBTB7B protein by antagonizing ubiquitin conjugation and degradation, this mechanism may be involved in CD4/CD8 lineage differentiation. Acetylates GABPB1, impairing GABPB1 heterotetramerization and activity. Acetylates PCK1 and promotes PCK1 anaplerotic activity. Acetylates RXRA and RXRG. Acetylates isoform M2 of PKM (PKM2), promoting its homodimerization and conversion into a protein kinase. Acetylates RPTOR in response to leucine, leading to activation of the mTORC1 complex. In addition to protein acetyltransferase, can use different acyl-CoA substrates, such as (2E)-butenoyl-CoA (crotonyl-CoA), butanoyl-CoA (butyryl-CoA), 2-hydroxyisobutanoyl-CoA (2-hydroxyisobutyryl-CoA), lactoyl-CoA or propanoyl-CoA (propionyl-CoA), and is able to mediate protein crotonylation, butyrylation, 2-hydroxyisobutyrylation, lactylation or propionylation, respectively. Acts as a histone crotonyltransferase.crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Histone crotonyltransferase activity is dependent on the concentration of (2E)-butenoyl-CoA (crotonyl-CoA) substrate and such activity is weak when (2E)-butenoyl-CoA (crotonyl-CoA) concentration is low. Also acts as a histone butyryltransferase.butyrylation marks active promoters. Catalyzes histone lactylation in macrophages by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription. Acts as a protein-lysine 2-hydroxyisobutyryltransferase.regulates glycolysis by mediating 2-hydroxyisobutyrylation of glycolytic enzymes. Functions as a transcriptional coactivator for SMAD4 in the TGF-beta signaling pathway. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. |
| Protein Name | Histone Acetyltransferase P300P300 HatE1a-Associated Protein P300Histone Butyryltransferase P300Histone Crotonyltransferase P300Protein 2-Hydroxyisobutyryltransferase P300Protein Lactyltransferas P300Protein Propionyltransferase P300 |
| Database Links | Reactome: R-HSA-1234158Reactome: R-HSA-1368082Reactome: R-HSA-156711Reactome: R-HSA-1912408Reactome: R-HSA-1989781Reactome: R-HSA-201722Reactome: R-HSA-210744Reactome: R-HSA-2122947Reactome: R-HSA-2173796Reactome: R-HSA-2197563Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-3134973Reactome: R-HSA-3214847Reactome: R-HSA-3371568Reactome: R-HSA-381340Reactome: R-HSA-3899300Reactome: R-HSA-400253Reactome: R-HSA-5250924Reactome: R-HSA-5617472Reactome: R-HSA-5621575Reactome: R-HSA-5689901Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6804114Reactome: R-HSA-6804758Reactome: R-HSA-6804760Reactome: R-HSA-6811555Reactome: R-HSA-8866907Reactome: R-HSA-8936459Reactome: R-HSA-8939243Reactome: R-HSA-8941856Reactome: R-HSA-8941858Reactome: R-HSA-8951936Reactome: R-HSA-9013508Reactome: R-HSA-9013695Reactome: R-HSA-9018519Reactome: R-HSA-9029569Reactome: R-HSA-9031628Reactome: R-HSA-918233Reactome: R-HSA-933541Reactome: R-HSA-9614657Reactome: R-HSA-9616222Reactome: R-HSA-9617629Reactome: R-HSA-9701898Reactome: R-HSA-9707616Reactome: R-HSA-9735871Reactome: R-HSA-9759194Reactome: R-HSA-9793380Reactome: R-HSA-9818027Reactome: R-HSA-9818028 |
| Cellular Localisation | CytoplasmNucleusChromosomeLocalizes To Active Chromatin: Colocalizes With Histone H3 Acetylated And/Or Crotonylated At 'Lys-18' (H3k18ac And H3k18crRespectively)In The Presence Of Alx1 Relocalizes From The Cytoplasm To The NucleusColocalizes With Rock2 In The NucleusLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Histone Acetyltransferase P300 antibodyAnti-P300 Hat antibodyAnti-E1a-Associated Protein P300 antibodyAnti-Histone Butyryltransferase P300 antibodyAnti-Histone Crotonyltransferase P300 antibodyAnti-Protein 2-Hydroxyisobutyryltransferase P300 antibodyAnti-Protein Lactyltransferas P300 antibodyAnti-Protein Propionyltransferase P300 antibodyAnti-EP300 antibodyAnti-P300 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
