• Western blot analysis of extracts of various cell lines, using ENO2 antibody (STJ114221) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells using ENO2 rabbit polyclonal antibody (STJ114221) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ENO2 rabbit polyclonal antibody (STJ114221) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Raji cells using ENO2 rabbit polyclonal antibody (STJ114221) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse brain cells using 3 Mu g ENO2 antibody (STJ114221). Western blot was performed from the immunoprecipitate using ENO2 antibody (STJ114221) at a dilution of 1:1000.

Anti-ENO2 antibody (195-434) (STJ114221)

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STJ114221

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ENO2 (195-434) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ENO2
Gene ID: 2026
Uniprot ID: ENOG_HUMAN
Immunogen Region: 195-434
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2 (NP_001966.1).
Immunogen Sequence: VIKDKYGKDATNVGDEGGFA PNILENSEALELVKEAIDKA GYTEKIVIGMDVAASEFYRD GKYDLDFKSPTDPSRYITGD QLGALYQDFVRDYPVVSIED PFDQDDWAAWSKFTANVGIQ IVGDDLTVTNPKRIERAVEE KACNCLLLKVNQIGSVTEAI QACKLAQENGWGVMVSHRSG ETEDTFIADLVVGLCTGQIK TGAPCRSERLAKYNQLMRIE EELGDEARFAGHNFRNPSV
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
Protein Name Gamma-Enolase
2-Phospho-D-Glycerate Hydro-Lyase
Enolase 2
Neural Enolase
Neuron-Specific Enolase
Nse
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Cellular Localisation Cytoplasm
Cell Membrane
Can Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) Form
Alternative Antibody Names Anti-Gamma-Enolase antibody
Anti-2-Phospho-D-Glycerate Hydro-Lyase antibody
Anti-Enolase 2 antibody
Anti-Neural Enolase antibody
Anti-Neuron-Specific Enolase antibody
Anti-Nse antibody
Anti-ENO2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance