• Western blot analysis of various lysates using EN2 Rabbit polyclonal antibody (STJ119584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EN2 antibody (250-333) (STJ119584)

SKU:
STJ119584

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EN2 (250-333) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EN2
Gene ID: 2020
Uniprot ID: HME2_HUMAN
Immunogen Region: 250-333
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 250-333 of human EN2 (NP_001418.2).
Immunogen Sequence: AFTAEQLQRLKAEFQTNRYL TEQRRQSLAQELSLNESQIK IWFQNKRAKIKKATGNKNTL AVHLMAQGLYNHSTTAKEGK SDSE
Protein Name Homeobox Protein Engrailed-2
Homeobox Protein En-2
Hu-En-2
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Engrailed-2 antibody
Anti-Homeobox Protein En-2 antibody
Anti-Hu-En-2 antibody
Anti-EN2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance