| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-EMI2 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in Mouse Ovary lysates (calculated MW of 78.4kDa according to NP_001025031.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Prelimin |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | FBXO43 |
| Gene ID: | 286151 |
| Uniprot ID: | FBX43_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_001025031.2 |
| Immunogen Sequence: | PGSAQSKRNLKRL |
| Post Translational Modifications | Phosphorylated on Ser-76, Thr-234 and Ser-334 in response to calcium, which is a prerequisite for ubiquitination and proteasomal degradation. Ubiquitinated in response to calcium, which promotes proteasomal degradation. |
| Function | Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Plays a vital role in modulating the ubiquitilation of CCNB1 and CDK1 during gametogenesis. |
| Protein Name | F-Box Only Protein 43Endogenous Meiotic Inhibitor 2 |
| Alternative Antibody Names | Anti-F-Box Only Protein 43 antibodyAnti-Endogenous Meiotic Inhibitor 2 antibodyAnti-FBXO43 antibodyAnti-EMI2 antibody |
Information sourced from Uniprot.org
