• Western blot analysis of extracts of various cell lines, using EMCN antibody (STJ110792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using Endomucin antibody (STJ110792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EMCN antibody (19-135) (STJ110792)

SKU:
STJ110792

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EMCN
Gene ID: 51705
Uniprot ID: MUCEN_HUMAN
Immunogen Region: 19-135
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 19-135 of human Endomucin (NP_057326.2).
Immunogen Sequence: NSTGVLEAANNSLVVTTTKP SITTPNTESLQKNVVTPTTG TTPKGTITNELLKMSLMSTA TFLTSKDEGLKATTTDVRKN DSIISNVTVTSVTLPNAVST LQSSKPKTETQSSIKTT
Tissue Specificity Expressed in heart, kidney and lung.
Post Translational Modifications Highly O-glycosylated. Sialic acid-rich glycoprotein.
Function Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.
Protein Name Endomucin
Endomucin-2
Gastric Cancer Antigen Ga34
Mucin-14
Muc-14
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Membrane
Consistently Localized To The Plasma Membrane And Less Abundantly To Golgi-Like Perinuclear Stacks
Isoform 2: Secreted
Alternative Antibody Names Anti-Endomucin antibody
Anti-Endomucin-2 antibody
Anti-Gastric Cancer Antigen Ga34 antibody
Anti-Mucin-14 antibody
Anti-Muc-14 antibody
Anti-EMCN antibody
Anti-EMCN2 antibody
Anti-MUC14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance