• Western blot analysis of A-431, using ELOVL4 antibody (STJ23530) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of C6 cells using ELOVL4 rabbit polyclonal antibody (STJ23530) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ELOVL4 rabbit polyclonal antibody (STJ23530) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-431 cells using ELOVL4 rabbit polyclonal antibody (STJ23530) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ELOVL4 rabbit polyclonal antibody (STJ23530) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ELOVL4 antibody (1-100) (STJ23530)

SKU:
STJ23530

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ELOVL4 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELOVL4
Gene ID: 6785
Uniprot ID: ELOV4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELOVL4 (NP_073563.1).
Immunogen Sequence: MGLLDSEPGSVLNVVSTALN DTVEFYRWTWSIADKRVENW PLMQSPWPTLSISTLYLLFV WLGPKWMKDREPFQMRLVLI IYNFGMVLLNLFIFRELFMG
Tissue Specificity Expressed in the retina and at much lower level in the brain. Ubiquitous, highest expression in thymus, followed by testis, small intestine, ovary, and prostate. Little or no expression in heart, lung, liver, or leukocates.
Post Translational Modifications N-glycosylated.
Function Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that catalyzes the synthesis of very long chain saturated (VLC-SFA) and polyunsaturated (PUFA) fatty acids that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.
Protein Name Elongation Of Very Long Chain Fatty Acids Protein 4
3-Keto Acyl-Coa Synthase Elovl4
Elovl Fatty Acid Elongase 4
Elovl Fa Elongase 4
Very Long Chain 3-Ketoacyl-Coa Synthase 4
Very Long Chain 3-Oxoacyl-Coa Synthase 4
Database Links Reactome: R-HSA-75876
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Elongation Of Very Long Chain Fatty Acids Protein 4 antibody
Anti-3-Keto Acyl-Coa Synthase Elovl4 antibody
Anti-Elovl Fatty Acid Elongase 4 antibody
Anti-Elovl Fa Elongase 4 antibody
Anti-Very Long Chain 3-Ketoacyl-Coa Synthase 4 antibody
Anti-Very Long Chain 3-Oxoacyl-Coa Synthase 4 antibody
Anti-ELOVL4 antibody

Information sourced from Uniprot.org

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