Anti-ELF5 antibody (40-150) (STJ29261)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ELF5 (40-150) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ELF5 |
| Gene ID | 2001 |
| Uniprot ID | ELF5_HUMAN |
| Immunogen Region | 40-150 |
| Immunogen Sequence | EEYYPAFEHQTACDSYWTSV HPEYWTKRHVWEWLQFCCDQ YKLDTNCISFCNFNISGLQL CSMTQEEFVEAAGLCGEYLY FILQNIRTQGYSFFNDAEES KATIKDYADSN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human ELF5 (NP_938195.1). |
Additional Info
| Tissue Specificity | Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, kidney and prostate. Weakly expressed in placenta and lung. Isoform 1 and isoform 2 are differentially expressed in different tissues. In the kidney, only isoform 1 was expressed, while prostate expressed both isoforms, with levels of isoform 2 being higher. Expression is up-regulated during keratinocyte differentiation. Several epithelial carcinoma cell lines showed lack of expression. |
| Function | Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation. Isoform 2 binds to DNA sequences containing the consensus nucleotide core sequence GGAAT. Transcriptionally activates SPRR2A and the parotid gland-specific PSP promoters. |
| Protein Name | Ets-Related Transcription Factor Elf-5E74-Like Factor 5Epithelium-Restricted Ese-1-Related Ets FactorEpithelium-Specific Ets Transcription Factor 2Ese-2 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Ets-Related Transcription Factor Elf-5 antibodyAnti-E74-Like Factor 5 antibodyAnti-Epithelium-Restricted Ese-1-Related Ets Factor antibodyAnti-Epithelium-Specific Ets Transcription Factor 2 antibodyAnti-Ese-2 antibodyAnti-ELF5 antibodyAnti-ESE2 antibody |
Information sourced from Uniprot.org